[BioC] Is it right?

[michael watson (IAH-C)]

This is (quite) common practice.  The pooled sample represents a common
reference for the treated samples.  A common reference experimental
design *can* have certain advantages over direct comparisons, such as
easy extensibility and ease of interpretation.  Pooling the control
samples simply ensures that the common reference is more generic
(hopefully) and lights up more spots (hopefully).

Mick

-----Original Message-----
From: Kaushik, Narendra K [mailto:n.kaushik at imperial.ac.uk] 
Sent: 16 September 2004 22:56
To: bioconductor at stat.math.ethz.ch
Subject: [BioC] Is it right?


Hi Folks, 
 
I have read an article on two color microarray hybridization. The
authors have pooled control samples (1), this mixture was lablled,
hybridized together with separately labelled treated (x) samples on x
microarrays ( Treated samples were not pooled). Do you think that is
good idea?
 
Narendra 
Imperial College of Medicine, 
London W2 1PG
UK
 
 

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