[BioC] VSN error : 'ny' does not seem to be resulting from vsn

Saurin Jani saurin_jani at yahoo.com
Tue Oct 19 22:19:37 CEST 2004


Hi,

I am using R-2.0 with develOK=TRUE. Here, esetSub2 is
my differentially expressed gene expression set.

>esetSub2X <- getIntensityMatrix(esetSub2);

>esetSub2Norm <- vsn(esetSub2,describe.preprocessing =

 FALSE);
vsn: 762 x 6 matrix (1 stratum). Please wait for 10
dots: ..........

> esetSub2Train <- esetSub2Norm[,4:6];
Error in validObject(.Object) : Invalid "phenoData"
object: FALSE

> traceback();
7: stop(paste("Invalid \"", Class, "\" object: ",
errors, sep = ""))
6: validObject(.Object)
5: initialize(value, ...)
4: initialize(value, ...)
3: new("phenoData", pData = pD, varLabels = vL)
2: phenoData(x)[j, , ..., drop = FALSE]
1: esetSub2Norm[, 4:6]

> vsnPlotPar(esetSub2Norm, "factors")
Error in vsnPlotPar(esetSub2Norm, "factors") :
        'ny' does not seem to be resulting from vsn.

> vsnPlotPar(esetSub2Norm, "offsets")
Error in vsnPlotPar(esetSub2Norm, "offsets") :
        'ny' does not seem to be resulting from vsn.

> traceback();
2: stop("'ny' does not seem to be resulting from
vsn.")
1: vsnPlotPar(esetSub2Norm, "offsets")


is something wrong in my expression set?

thank you,
Saurin



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