[BioC] limma question
Julia Engelmann
julia.engelmann at biozentrum.uni-wuerzburg.de
Wed Mar 10 08:33:02 MET 2004
Hello all,
I am using limma on Affymetrix data and I read and followed the User's Guide
so far, but one thing is still not clear to me:
Are the M-Values returned by the toptable- command estimated log2-fold
changes? I know typing "?toptable" in R says "M: estimate of the effect or
the contrast", but I don't understand what that means.
If they aren't, where can I get fold changes for my data?
I have 4 untreated and 4 treated experiments, my R code is:
design<- cbind(c(1,1,1,1,1,1,1,1),c(0,0,0,0,1,1,1,1))
fit <- lm.series(exprs(Set),design)
eb <- ebayes(fit)
result <-toptable(coef=2,number=300,genelist=data,fit=fit,adjust="fdr")
Thanks a lot for your help,
Julia
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