[BioC] affy 1 cycle vs 2 cycle labeling kit

[Michael Seewald]

Dear Fai,

I you have homogenous datasets with either only 1x amplified samples or only
2x amplified samples, that's ok. If you mix them, you will not be able to
compare them. They will form separate clusters.

Best regards,
Michael


On Fri, 11 Jun 2004, YUK FAI LEUNG wrote:

> Hi there,
>
> I wonder if anyone have compared the data correlation between the same
> sample labeled by Affy's one cycle labeling and two cycle labeling kit?
> The amount of some of my pooled samples is in the lower end of the one
> cycle labeling kit. Life would be easier if I use the two cycle kit. But
> as we all know, more processing steps and amplication may lead to
> variation. I am not sure whether I want to use the two cycle kit or just
> collect more samples. Any thoughts?
>
> Regards,
> Fai