[BioC] RE:proper pooling design

[YUK FAI LEUNG]

Hi,

There is another issue that comes up from my discussion with some people 
about my earlier question on pooling design. It is very likely that I 
can't collect all the samples in one day, not even the pooled biological 
replicates. I have heard many people say that the time of collection 
(i.e. day) has a great effect on resulting clustering etc. data 
analysis. I guess this might be true in some cases,  although I haven't 
looked into this factor seriously. I would if anyone has any experiences 
on this.

If this effect is true in my case, then the variation among the 
biological replicates might be larger than it should be, which I want to 
avoid. Could someone suggest a sequence of sample collection based on a 
proper statisical design which can avoid/minimize such collection-time 
variation on data analysis?

Thanks!

Best regards,
Fai
-- 
Yuk Fai Leung
Department of Molecular and Cellular Biology
Harvard University
BL 2079, 16 Divinity Avenue
Cambridge, MA 02138
Tel: 617-495-2599
Fax: 617-496-3321
email: yfleung at mcb.harvard.edu; yfleung at genomicshome.com
URL: http://genomicshome.com