[BioC] P calls (VSN and RMA)

w.huber at dkfz-heidelberg.de w.huber at dkfz-heidelberg.de
Tue Jan 13 14:25:24 MET 2004


Hi Petra,
two questions:

> I used VSN data and did a filtering step ...

Did you use just the PM values or PM and MM (and if so, how)?

> Thus, the transition between sensitivity and selectivity occurs at
> intensity values between 7.4 and 7.9.

>From what other people have told me, I'd expect that there is not a
universal value, but that it would be different for different probe
sequences (according to the distribution of C, G, T, and A across the
positions 1..25). Also, the exact value would depend on scanner settings
etc. .... Opinions?

Best wishes
 Wolfgang

-------------------------------------
Wolfgang Huber
Division of Molecular Genome Analysis
German Cancer Research Center
Heidelberg, Germany
Phone: +49 6221 424709
Fax:   +49 6221 42524709
Http:  www.dkfz.de/abt0840/whuber



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