[BioC] t test

[Binita Dutta]

Hi All,

I have done a very simple cDNA microarray experiment (Wild type -Cy5 
Vs  Mutant variety-Cy3 and color flip expriment on our mouse arrays). 
This  experiment was repeated and i have results of all these four 
experiment.  Using Marray norm i could normalise the data and have exported 
M and A  values on excel sheet. However, I need your suggestions to 
interpret the  result using  R package (Genes  which are differentially 
expressed in  mutant type compared to the wild type). I wanted to use 
multtest package (which probaly is not the right package for these 
experiments) but have not been able to get anything out from it yet.

In our lab we calculate normalised R and G values (from M and A values)and 
then do t test in microsof excel to check for differentially expressed 
genes. But I  want to use biocondutor to check differentially expressed genes.

Please let me know if it is possible to analyse these data using 
bioconductor program.

Binita







Dr. Binita Dutta
MicroArray Facility(MAF)
UZ Gasthuisberg
Onderwijs en Navorsing
Herestraat 49
3000 Leuven
Belgium