[BioC] limmaGUI: Input files question!
Marcelo Luiz de Laia
mlaia at fcav.unesp.br
Fri Oct 17 14:51:35 MEST 2003
I need analyse the macroarrays.
Im my case, I dont have Cy3 and Cy5. I have 2 nylon membranes for each
replicaties: one control and one treated. In my experiments, I have 3
replicaties. The softwere image analyze have 1 out put files for each 2
nylon membrane with 3 colunms for control and 3 colunms for treated, like
this (Ctrl=control, Data=treated):
Ctrl Ctrl Ctrl Data Data Data
VOL Bkgd sVOL VOL Bkgd sVOL
A - 1 : 1 (1) 14.3 13.7 0.5 481.0 420.3 60.7
- In the Targets file have four essential columns (I read it in the limmaGUI
man in web site). The colunms Cy3 and Cy5 are essential for limmaGUI?
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