[BioC] Re: limma package

Hua Weng hweng at bmb-fs1.biochem.okstate.edu
Thu Oct 2 17:28:09 MEST 2003


Hi, all:

I'm trying to read in intensity data in limma package. But I get the following error:

> files <- dir(pattern="*\\.gpr")
> RG <- read.maimages(files, source="genepix")
Error in read.maimages(files, source = "genepix") :
        Cannot find column heading in image output file

I don't know what does this error mean. The gpr files I try to load are from GenePix Pro 4.0.1.17, the wavelength I chose for Cy5/Cy3 are 647/546. Do I need to specify these information and How?

Thanks,
Hua
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