[BioC] expresso and mas5 scaling
johnston at pat.biochem.ucl.ac.uk
johnston at pat.biochem.ucl.ac.uk
Mon Jul 7 18:34:05 MEST 2003
Hi,
I'm trying out different combinations of normalisation and expression summaries
on some affy data using expresso and am confused by the comment in the expresso
help file about having to scale mas-summarised values after running expresso.
Is this just for compatibility with mas software or are some of the
normalisation methods not applicable to probe-level data? Does the
affy.scalevalue.exprSet method just perform the eqivalent of the expresso
constant normalisation, but on expression summaries rather than probe-level
data or am I missing something?
Apologies if this is a dumb question
Cassxx.
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