[BioC] expresso and mas5 scaling

johnston at pat.biochem.ucl.ac.uk johnston at pat.biochem.ucl.ac.uk
Mon Jul 7 18:34:05 MEST 2003


Hi,

I'm trying out different combinations of normalisation and expression summaries 
on some affy data using expresso and am confused by the comment in the expresso 
help file about having to scale mas-summarised values after running expresso. 
Is this just for compatibility with mas software or are some of the 
normalisation methods not applicable to probe-level data? Does the 
affy.scalevalue.exprSet method just perform the eqivalent of the expresso 
constant normalisation, but on expression summaries rather than probe-level 
data or am I missing something?

Apologies if this is a dumb question

Cassxx.

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