[BioC] Construction an instance of class marrayRaw
Jean Yee Hwa Yang
jean@biostat.ucsf.edu
Sun, 23 Feb 2003 15:15:16 -0800 (PST)
Hi Hinnerk,
It is likely that matrix(red) isn't a M by N matrix. Check
dim(matrix(red))
class(matrix(red))
In addition, try the following:
exp2raw <- new("marrayRaw", maRf=as.matrix(red), maGf=as.matrix(green))
exp2raw
To get a matrix of log-ratios, use
maM(exp2raw)
Hope this helps
Jean
> GeneM 2. 4. 5.
>
> Then I tried the following:
>
> > infos <- new("marrayInfo", maLabels=dimnames(green)[[1]])
> > exp2raw <- new("marrayRaw", maRf=matrix(red), maGf=matrix(green),
> maTargets=infos)
>
> Now looking at the object reveals that something went wrong: (btw: I was
> having 10 chips)
>
> > exp2raw
> Pre-normalization intensity data: Object of class marrayRaw.
>
> Number of arrays: 1 arrays.
>
> A) Layout of spots on the array:
> Array layout: Object of class marrayLayout.
>
> Total number of spots:
> Dimensions of grid matrix: rows by cols
> Dimensions of spot matrices: rows by cols
>
> Currently working with a subset of spots.
>
> Control spots:
>
>
> Notes on layout:
>
>
> B) Samples hybridized to the array:
> Object of class marrayInfo.
>
> [1] "A" "B" "C" "D" "E" "F" "G" "H" "I" "K"
> ...
>
> Number of labels: 348
> Dimensions of maInfo matrix: rows by columns
>
> Notes:
>
>
> C) Summary statistics for log-ratio distribution:
> Min. 1st Qu. Median Mean 3rd Qu. Max. NA's
> [1,] NA NA NA NA NA NA 10
>
> D) Notes on intensity data:
>
> ----------
> What am I missing here. There is apparently only one chip recognized,
> even though I supplied a matrix with 10 columns. Do I need to specify a
> layout? Right now I don't really care about spacial effects, that's why
> I didn't bother playing with it. I hope you can help me to bring me on
> the right track.
>
> Cheers,
> Hinnerk
>
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