[BioC] Construction an instance of class marrayRaw
Hinnerk Boriss
boriss@izbi.uni-leipzig.de
Sun, 23 Feb 2003 23:34:18 +0100
Hi,
I am trying to construct an object of class marrayRaw from scratch. I
have red and green intensities stored in data frames that look something
like this:
G_name chip1 chip2 ... chipN
Gene1 0.1 2. 3.
.
.
.
GeneM 2. 4. 5.
Then I tried the following:
> infos <- new("marrayInfo", maLabels=dimnames(green)[[1]])
> exp2raw <- new("marrayRaw", maRf=matrix(red), maGf=matrix(green),
maTargets=infos)
Now looking at the object reveals that something went wrong: (btw: I was
having 10 chips)
> exp2raw
Pre-normalization intensity data: Object of class marrayRaw.
Number of arrays: 1 arrays.
A) Layout of spots on the array:
Array layout: Object of class marrayLayout.
Total number of spots:
Dimensions of grid matrix: rows by cols
Dimensions of spot matrices: rows by cols
Currently working with a subset of spots.
Control spots:
Notes on layout:
B) Samples hybridized to the array:
Object of class marrayInfo.
[1] "A" "B" "C" "D" "E" "F" "G" "H" "I" "K"
...
Number of labels: 348
Dimensions of maInfo matrix: rows by columns
Notes:
C) Summary statistics for log-ratio distribution:
Min. 1st Qu. Median Mean 3rd Qu. Max. NA's
[1,] NA NA NA NA NA NA 10
D) Notes on intensity data:
----------
What am I missing here. There is apparently only one chip recognized,
even though I supplied a matrix with 10 columns. Do I need to specify a
layout? Right now I don't really care about spacial effects, that's why
I didn't bother playing with it. I hope you can help me to bring me on
the right track.
Cheers,
Hinnerk