[BioC] How to use spiked controls

[Naomi Altman]

Spiked controls can be used to:

1) Determine the success of the hybridization.
2) Center arrays when there is reason to think that there may be a large 
percentage of genes that express differently on different arrays
3) Used in a titration series, the spiked controls can be used to at least 
somewhat calibrate the expression levels from the array with the actual RNA 
concentration.

Regarding the last idea, I have not yet ploughed through all of the 
Bioconductor document - is there a routine to assist with this type of 
calibration?

At 08:23 PM 12/15/2003, zhao luo wrote:
>Dear friends,
>
>I'm doing a project about microarray data normalization, and Arabidopsis 
>thaliana gene is used as spiked control. But  I'm very confused to use 
>spiked controls, please give me some idea about it.
>Thanks a lot
>
>/Zhao
>
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Naomi S. Altman                                814-865-3791 (voice)
Associate Professor
Bioinformatics Consulting Center
Dept. of Statistics                              814-863-7114 (fax)
Penn State University                         814-865-1348 (Statistics)
University Park, PA 16802-2111