Martin Morgan mtmorgan at fhcrc.org
Thu Sep 8 04:28:23 CEST 2011

On 09/07/2011 11:34 AM, wang peter wrote:
> i replaced the low quality position with "N"
> and then trimmed N from 5' and 3'
> i got the reads which only contain the "N" in the middle
> if i want to find those reads before replacement by "N"
> WHAT CAN I DO?

Hi Peter --

Not sure what you mean, probably not this

startsWithN <- as.character(narrow(sread(rfq), 1, 1)) == "N"
endsWithN <-
middleN <-
!startsWithN & !endsWithN & alphabetFrequency(sread(rfq))[,"N"] != 0
rfq[!middleN]

I think below your code can be simplified with

at <- as(quality(read), "matrix") < qualityCutoff

Martin

>
> THX
> such is coding
>
>
> nCount<-alphabetFrequency(seqs)[,"N"]
> nDist<- table(nCount)
>
> qualityCutoff<- 20
> myqual_mat<- matrix(charToRaw(as.character(unlist(qual))),
> nrow=length(qual), byrow=TRUE) # convert quality score to matrix
> at<- myqual_mat<
> charToRaw(as.character(PhredQuality(as.integer(qualityCutoff))))
> letter_subject<- DNAString(paste(rep.int("N", width(seqs)[1]),
> collapse=""))
> letter<- as(Views(letter_subject, start=1, end=rowSums(at)),
> "DNAStringSet")
> injectedseqs<- replaceLetterAt(seqs, at, letter)
>
> trimmed<-trimLRPatterns(Rpattern = letter_subject, Lpattern =
> letter_subject,subject = injectedseqs)
> nCount<-alphabetFrequency(trimmed)[,"N"]
> nDist<- table(nCount)
>
> thank u
>
> shan gao
>
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>
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