[Bioc-devel] GSE62944, empty EH164 and EH165

Ludwig Geistlinger Ludwig.Geistlinger at sph.cuny.edu
Fri Dec 15 19:56:34 CET 2017


Great! Thanks for resolving this so fast. Ludwig


________________________________
From: Shepherd, Lori <Lori.Shepherd at RoswellPark.org>
Sent: Friday, December 15, 2017 1:48 PM
To: Ludwig Geistlinger; bioc-devel at r-project.org
Subject: Re: GSE62944, empty EH164 and EH165


Thank you for bringing this to our attention.


The resources were created with a previous version of SummarizedExperiment that were not compatible with the current version.


New resources have been added and are visible in both release 3.6 and devel 3.7. Please note the new EH_id's:



> library(ExperimentHub)

> hub <- ExperimentHub()
snapshotDate(): 2017-10-30
> gse <- AnnotationHub::query(hub, "GSE62944")
> gse
ExperimentHub with 3 records
# snapshotDate(): 2017-10-30
# $dataprovider: GEO
# $species: Homo sapiens
# $rdataclass: SummarizedExperiment, ExpressionSet
# additional mcols(): taxonomyid, genome, description,
#   coordinate_1_based, maintainer, rdatadateadded, preparerclass, tags,
#   rdatapath, sourceurl, sourcetype
# retrieve records with, e.g., 'object[["EH1"]]'

           title
  EH1    | RNA-Sequencing and clinical data for 7706 tumor samples from The...
  EH1043 | RNA-Sequencing and clinical data for 9246 tumor samples from The...
  EH1044 | RNA-Sequencing and clinical data for 741 normal samples from The...

> x = gse[["EH1043"]]
> x
class: SummarizedExperiment
dim: 23368 9264
metadata(0):
assays(1): CancerRaw
rownames(23368): 1/2-SBSRNA4 A1BG ... ZZZ3 tAKR
rowData names(0):
colnames(9264): TCGA-G9-A9S0-01A-11R-A41O-07
  TCGA-E1-5318-01A-01R-1470-07 ... TCGA-A7-A0DC-01A-11R-A00Z-07
  TCGA-A5-A0VQ-01A-11R-A104-07
colData names(549): bcr_patient_uuid bcr_patient_barcode ...
  lymph_nodes_aortic_pos_total CancerType
> dim(x)
[1] 23368  9264


> x = gse[["EH1044"]]
> x
class: SummarizedExperiment
dim: 23368 741
metadata(0):
assays(1): NormalRaw
rownames(23368): 1/2-SBSRNA4 A1BG ... ZZZ3 tAKR
rowData names(0):
colnames(741): TCGA-K4-A3WV-11A-21R-A22U-07
  TCGA-49-6742-11A-01R-1858-07 ... TCGA-BH-A0H5-11A-62R-A115-07
  TCGA-22-5489-11A-01R-1635-07
colData names(2): sample type
> dim(x)
[1] 23368   741


Cheers,



Lori Shepherd

Bioconductor Core Team

Roswell Park Cancer Institute

Department of Biostatistics & Bioinformatics

Elm & Carlton Streets

Buffalo, New York 14263

________________________________
From: Bioc-devel <bioc-devel-bounces at r-project.org> on behalf of Ludwig Geistlinger <Ludwig.Geistlinger at sph.cuny.edu>
Sent: Thursday, December 14, 2017 3:01:56 PM
To: bioc-devel at r-project.org
Subject: [Bioc-devel] GSE62944, empty EH164 and EH165

Hi,


I'm trying to obtain the most recent version of GSE62944 from ExperimentHub via


> library(ExperimentHub)

> hub <- ExperimentHub()

> gse <- AnnotationHub::query(hub, "GSE62944")


> gse
ExperimentHub with 3 records
# snapshotDate(): 2017-10-30
# $dataprovider: GEO
# $species: Homo sapiens
# $rdataclass: SummarizedExperiment, ExpressionSet
# additional mcols(): taxonomyid, genome, description,
#   coordinate_1_based, maintainer, rdatadateadded, preparerclass, tags,
#   rdatapath, sourceurl, sourcetype
# retrieve records with, e.g., 'object[["EH1"]]'

          title
  EH1   | RNA-Sequencing and clinical data for 7706 tumor samples from The...
  EH164 | RNA-Sequencing and clinical data for 9246 tumor samples from The...
  EH165 | RNA-Sequencing and clinical data for 741 normal samples from The...


Retrieving the original (outdated) dataset works fine:

> x <- gse[["EH1"]]

> dim(x)
Features  Samples
   23368     7706


However,  obtaining the most recent version of the data via


> x <- gse[["EH164"]]


gives me:


> dim(x)
NULL


> class(x)
[1] "SummarizedExperiment0"
attr(,"package")
[1] "SummarizedExperiment"

> str(x)
Formal class 'SummarizedExperiment0' [package "SummarizedExperiment"] with 0 slots
 Named list()


It would be great if this could be updated.


Thanks a lot,

Ludwig


--
Dr. Ludwig Geistlinger
CUNY School of Public Health



> sessionInfo()
R version 3.4.2 (2017-09-28)
Platform: x86_64-apple-darwin15.6.0 (64-bit)
Running under: macOS High Sierra 10.13.1

Matrix products: default
BLAS: /Library/Frameworks/R.framework/Versions/3.4/Resources/lib/libRblas.0.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/3.4/Resources/lib/libRlapack.dylib

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

attached base packages:
[1] stats4    parallel  stats     graphics  grDevices utils     datasets
[8] methods   base

other attached packages:
 [1] SummarizedExperiment_1.8.0 DelayedArray_0.4.1
 [3] matrixStats_0.52.2         GenomicRanges_1.30.0
 [5] GenomeInfoDb_1.14.0        IRanges_2.12.0
 [7] S4Vectors_0.16.0           GSE62944_1.6.0
 [9] GEOquery_2.46.8            Biobase_2.38.0
[11] ExperimentHub_1.4.0        AnnotationHub_2.10.1
[13] BiocGenerics_0.24.0

loaded via a namespace (and not attached):
 [1] Rcpp_0.12.14                  XVector_0.18.0
 [3] BiocInstaller_1.28.0          compiler_3.4.2
 [5] bindr_0.1                     zlibbioc_1.24.0
 [7] bitops_1.0-6                  tools_3.4.2
 [9] digest_0.6.12                 bit_1.1-12
[11] lattice_0.20-35               RSQLite_2.0
[13] memoise_1.1.0                 tibble_1.3.4
[15] pkgconfig_2.0.1               rlang_0.1.4
[17] Matrix_1.2-12                 shiny_1.0.5
[19] DBI_0.7                       curl_3.0
[21] yaml_2.1.14                   bindrcpp_0.2
[23] GenomeInfoDbData_0.99.1       xml2_1.1.1
[25] httr_1.3.1                    dplyr_0.7.4
[27] hms_0.3                       grid_3.4.2
[29] bit64_0.9-7                   glue_1.2.0
[31] R6_2.2.2                      AnnotationDbi_1.40.0
[33] limma_3.34.3                  purrr_0.2.4
[35] tidyr_0.7.2                   readr_1.1.1
[37] blob_1.1.0                    magrittr_1.5
[39] htmltools_0.3.6               assertthat_0.2.0
[41] mime_0.5                      interactiveDisplayBase_1.16.0
[43] xtable_1.8-2                  httpuv_1.3.5
[45] RCurl_1.95-4.8


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