[Bioc-devel] ExpressionSet for qRT-PCR

[James W. MacDonald]

Vincent Carey 525-2265 wrote:
> while this topic is being aired, can i ask about design
> considerations?  i find that people using RT-PCR are
> prone to putting their replicates next to each other on the
> plates; if they have multiple plates they may not distribute
> samples from different conditions systematically.
> perhaps the platforms you are describing already
> address this?

The SuperArrays are a commercial product, so all plates of the same type 
will have the genes being interrogated arranged consistently from one 
plate to another. The design is based on a single sample per plate 
(i.e., one sample, multiple genes) rather than the convention with most 
PCR runs of multiple samples, single (or very few) genes.



-- 
James W. MacDonald
University of Michigan
Affymetrix and cDNA Microarray Core
1500 E Medical Center Drive
Ann Arbor MI 48109
734-647-5623



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