[Bioc-devel] FCS3.0 DiVa files?

[Florian Hahne]

Hi Byron,
As far as I know these files don't seem to adhere to the specifications
in the FCS 3.0 format. I had one example file which I read in using a
number of different software tools and almost all of them produced
different results.
readFCS could read the file without errors and the plots of the data
looked ok. The only problem is that the absolute values are not the same
for the different software, but the measurement relations are the same,
i.e. the data is on different scales. And since I don't know what the
correct values are (and are there "correct" values at all? In the end
these are just arbitrary dimensionless numbers that somehow reflect the
fluorescence intensities...) I didn't know how to proceed.
Please feel free to take a stab at the import if you think that is a
problem or send me one or two of your files along with some info about
the expected range so I can give it another try.
Cheers,
Florian

Byron Ellis schrieb:
> Hi all,
>
> I remember a couple of months ago there was a brief discussion about
> FCS3.0 files, in particular DiVA files recorded in floating point. Was
> there ever any progress made on reading those in? I took a look
> through the logs, but I may have missed something. I suddenly find
> myself working with an LSR2 using DiVa that generates a lot of these
> files. :-)
>
> If not, I'll take a stab at it since I need to read these files sooner
> rather than later.
>
>   


-- 
Florian Hahne
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