Hi All,

I have 46 replicate trees I sampled in a 2 year survey. 
My objective is: To estimate the proportion of variation among-trees w/in year, and proportion variation due to among year and tree interaction.

Response	Year	Tree
23	A	1
12	A	2
54	A	3
23	B	1
12	B	2
9	B	3
The above is an example of my dataset


Circularity was violated, so instead of running a Repeated Measures I am running a mixed effects model. With trees as random and year as fixed. I'm then running a variance components analysis.

Q1. Is this a good way to test the above questions?

My model is this: lme <-lme(response~year,random=~1|tree/year,df)

I am not getting an interaction term here, nor do I when I run an aov model using tree*year as a factor.

Q2. I'm wondering why I'm not coming up with an interaction term for year and tree using aov or lme even though my variance comp. analysis says that most of the variation (55%) is due to year and tree interaction.

Thanks!!




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