Hi folks,

First - my apologies: I just discovered that my replies (from June 21st and
yesterday) to your replies to this thread did not go through for some
reason (a quick search of the archives revealed this). My thanks, replies,
and additional q's are pasted below:

Thanks for replying so quickly Alain – it’s much appreciated. To follow-up
on your comments:

-          Re: Spatial Autocorrelation  - I have dealt with spatial
autocorrelation in the past, though with continuous log-normal data (no
random effects - hence I used a spatial autoregressive model). I have
mentioned the likelihood of spatial autocorrelation in the residuals to my
employer/supervisor; however, he has advised that we proceed with the model
without accounting for autocorrelation, expecting that a large part it may
be explained by the environmental variables (which are no doubt clustered)
once the model is fitted. I’m skeptical, as some of these species also
might seek “safety in numbers” selecting sites based on the abundance of
conspecifics nearby, and large flocks at a given site are likely to utilize
habitat at neighboring sites as well (if suitable). We shall see!

-          Re: random effect in the binomial process of a ZIP - don’t I
have to include this, given the repeated measures?

Thanks to everyone else as well for your input. After reading your
responses, and diving into the lit a little more, you've convinced me that
MCMC is the way to go. However, I now have a few more quick (hopefully?)
questions:
- Because I'm a tad afraid of WinBugs, I decided to look at MCMCglmm as
well. I noticed that the course notes for MCMCglmm state that “*As is often
the case the parameters of the zero-inflation model mixes poorly… Poor
mixing is often associated with distributions that may not be zero-inflated
but instead over-dispersed.*”  Am I correct in thus assuming that if the
data are indeed zero-inflated, “poor mixing” is not a problem? Or might
this also arise through other means?

- Is there an advantage to using MCMCglmm versus winBUGS or vice versa? It
seems either one will take some time to correctly code/specify, so I might
as well go the route that makes the most sense/is more highly recommended.

- And most importantly: As I mentioned in my original message, we had
wanted to compare competing hypotheses for what shoreline attributes
influence shorebird distributions, and to then use MMI in prediction;
however, I’ve read that DIC is not recommended for mixed effects models
(even though MuMIn accepts MCMCglmm output). According to a post by Jarrod
Hadfield, this is especially true for non-Gaussian data because the level
of focus is on the sampled observations (i.e., for “*observations (y) on
children within schools...DIC would be focused at "can we predict how many
times *these* children miss the bus*"*)*. What are my options then for
model comparison/selection and prediction? Recall that we want to estimate
the total abundance of each shorebird species within the entire study
region (with confidence intervals). I'm really stuck here...

Thanks in advance... this is a huge statistical leap for me.

Cheers,
Jenn

On Thu, Jun 21, 2012 at 8:43 PM, Paul Johnson <pauljohn32@gmail.com> wrote:

> Dear Jennifer:
> Response below
>
> On Wed, Jun 20, 2012 at 5:32 PM, Jennifer Barrett
> <jenn.s.barrett@gmail.com> wrote:
> > Hi folks,
> >
> >
> > I’m looking for some guidance in regards to zero-inflated models with
> > repeated measures (i.e., random effect for site). My first question is
> more
> > of a statistical one, while the second is related to R packages.
> Apologies
> > for the long post; however, I want to make sure my concerns/questions are
> > clear!
> >
> >
> > Our project and dataset:
> >
> >
> > - The aim of our project is to 1) examine associations between shoreline
> > habitat characteristics and the abundance of several shorebird species;
> and
> > 2) estimate the total abundance of each shorebird species within the
> entire
> > study region based on the models from 1) above, with confidence
> intervals.
> > Note that we will be using an information theoretic approach for 1)
> above,
> > and would like to use MMI for 2).
> >
> > - Our response dataset consists of counts of shorebirds at >150 coastal
> > sites, conducted on the second Sunday of each month between the months of
> > Oct-March, over 10 years; however, not every site was surveyed in all
> > months (we’ve limited our dataset to those with a minimum of 3 counts in
> a
> > year).  Our response variable is thus the number of birds counted in a
> > given month/year at a given site. Note that we plan to model each year
> > separately.
> >
> > -  The habitat dataset consists of shoreline units within our entire
> study
> > region, with each unit characterized by exposure, substrate type...etc.
> > Using GIS, we’ve measured the length of shoreline belonging to shoreline
> > categories (e.g., sand, rock, mud) within each survey site, the average
> > exposure for the site, and other continuous attributes, as well as one
> > presence/absence covariate.
> >
> > - Initial exploratory analysis has shown that the counts are
> zero-inflated.
> > While there may be some false zeros in our dataset (i.e., observer
> error),
> > the source of the zero-inflation is likely preference of shorebirds for
> > particular sites with particular features and avoidance of others (i.e.,
> > true zeros or “structural zeros”). Some zeros likely also arise because
> the
> > species does not saturate its habitat (i.e., habitat suitable, but
> > unoccupied – also a “true” zero), though again, the majority of the zeros
> > are likely structural.
> >
> >
> > Onto my questions:
> >
> >
> > 1) I’ve been reading through the literature to decide what type of model
> > would best be suited for our dataset and questions. While all articles
> seem
> > to agree that the choice of a model needs to consider the source of
> excess
> > zeros, they seem to contradict one another in regards to what zeros are
> > being modeled in each component of a zero-inflated mixture model. Note
> that
> > I am not considering a two-part (i.e., conditional) model, because I do
> not
> > believe that all zeros arise from the occupancy process (as per Joseph et
> > al. 2009 and as noted above, zero abundance can occur by chance in our
> > system). Examples:
> >
> >
> > - Martin et al. (2005) state that when zero inflation is due to true
> zeros,
> > two-part or mixture models (ZIP or ZINB) are recommended, and that when
> > zero inflation is due to false zeros, a ZIB mixture model is recommended;
> > however, when zero inflation is due to both excess true and false zeros,
> a
> > Bayesian framework may be used, though there is no formal discussion in
> the
> > literature. NOTE: Since this article was published, Royle’s N-mixture
> model
> > has addressed this issue; however, I cannot use this approach as my data
> do
> > not meet the assumption of a closed population during the study period.
> >
> > - In contrast to Martin et al. (2005), Potts and Elith (2006) state that
> > the zero-inflated mixture model structure implies that zero observations
> > arising from the zero process are true negative observations, and that
> > those arising from the Poisson process are false negative observations
> “that
> > is, the habitat is suitable, but unoccupied” (p.155). However, on the
> > previous page, they defined false negative as “attributable to
> experimental
> > design… or observer error”, and habitat that is “suitable, but
> unoccupied”
> > as a true negative, so I'm not sure which type of zero observation they
> are
> > really referring to here for the Poisson process.
> >
> > - In contrast to both sources above, Zuur et al. (2009) state that in a
> ZIP
> > or ZINB, zeros are modeled as coming from two processes – the binomial
> > process, which models only false zeros (observer, design, and survey
> error)
> > and the Poisson (or Negbin) process  which models the true zeros and
> > counts. This is the opposite of what was stated by Potts and Elith.
> >
> > - Finally, I’ve read other sources which state that ZIPs simply treat the
> > population as a mixture, with one set of subjects having a zero response
> –
> > in other words, there is no mention of whether the zero process is
> modeling
> > the “true” or “false” zeros.
> >
> >
> > Thinking about my system: there are a bunch of sites where the birds (of
> a
> > given species) never go (habitat is unsuitable), and a bunch where they
> do
> > go with varying levels of abundance (habitat is suitable, but come sites
> > are more favored than others, based on habitat features). Following the
> > last bullet above, a site that is suitable may have a count of zero
> simply
> > because the species wasn’t present there on the survey day (i.e., true
> zero
> > occurring by chance). Given the contradicting information above, and the
> > consensus on the importance of considering the source of zeros in model
> > selection, I would very much appreciate if someone could clear this up
> for
> > me - or let me know if I'm completely missing something here? Perhaps
> this
> > question should be posed on a stats forum, but given question 2 below, I
> > thought I'd try here first.
> >
> >
> > 2) Assuming that I’m on the right track with a ZIP, is there a package I
> > can use to model a ZIP with a random effect for site? I looked at
> glmmADMB;
> > however, the zero inflation can only be modeled as a constant. This
> doesn’t
> > make sense for my system, as the zero-inflation will be a function of
> > habitat covariates (see above). Likewise, glmmPQL is not an option, as
> this
> > method does not yield log-likelihoods (and thus no AIC). I’m also
> thinking
> > that the random effect will have to be included in the zero process as
> well
> > – is this right?
> >
> Some of your jargon is unfamiliar to me--"true" and "false" zeros. I
> suppose a false zero would be the result of a "hurdle process" (as in
> the pscl package).  I've not seen a hurdle model joined in the same
> with a zero-inflation model.  Certainly not with "random effects"
> apart from the inflated zeros.
>
> Although I do not believe there is an ML solution for your problem
> within easy reach. However, there are Bayesian answers. Please see the
> package MCMCglmm.  It has a very well done pair of vignettes.
>
> MCMCglmm has a ZIP family option, and you can add random effects.
> Jarod Hadfield has been a regular contributor here and I think if you
> post your working example code he and others will be glad to help out.
>
> pj
>
>
>
> --
> Paul E. Johnson
> Professor, Political Science    Assoc. Director
> 1541 Lilac Lane, Room 504     Center for Research Methods
> University of Kansas               University of Kansas
> http://pj.freefaculty.org            http://quant.ku.edu
>



-- 
Jennifer Barrett, BSc., MRM
Research Associate
Centre for Wildlife Ecology
Simon Fraser University

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