[R-sig-ME] zero variance query

Christine Griffiths Christine.Griffiths at bristol.ac.uk
Tue Jun 2 11:45:40 CEST 2009


Dear Emmanuel and Ben

Many thanks for your advice. Unfortunately, I don't think that I can offset 
with log(area), given that each area is the same. My rationale for 
converting to m2 was to standardise abundances to 1 m2 as I have other 
parameters which were measured to different areas. I had previously 
attempted to normalise my data by logging but felt that it did not improve 
the distribution. I just hadn't tried it in my modelling. Logging my count 
data dramatically improved the fit of the model (AIC 116.7 v 312.5), 
however the variance still remains low. Does this appear acceptable?
Furthermore, can I assess model fit of different transformations of the 
same dataset using AIC values, i.e. compare log(Count) and inverse 
transformed Count?

lncount<-log(Count+1)
m1<-m1<-lmer(lncount~Treatment+(1|Month)+(1|Block),family=quasipoisson)
summary(m1)
Generalized linear mixed model fit by the Laplace approximation
Formula: lncount ~ Treatment + (1 | Month) + (1 | Block)
   AIC   BIC logLik deviance
 116.7 135.1 -52.33    104.7
Random effects:
 Groups   Name        Variance   Std.Dev.
 Month    (Intercept) 1.8937e-14 1.3761e-07
 Block    (Intercept) 3.5018e-02 1.8713e-01
 Residual             3.9318e-01 6.2704e-01
Number of obs: 160, groups: Month, 10; Block, 6

Fixed effects:
                   Estimate Std. Error t value
(Intercept)         -0.4004     0.1239  -3.232
Treatment2.Radiata   0.4596     0.1305   3.522
Treatment3.Aldabra   0.4295     0.1334   3.220

Correlation of Fixed Effects:
            (Intr) Trt2.R
Trtmnt2.Rdt -0.581
Trtmnt3.Ald -0.577  0.530

I used quasipoisson as my data is overdispersed. It was further improved by 
an inverse transformation (AIC 43.54). Again I have small variances.

invcount<-1/(Count+1)
m3<-lmer(invcount~Treatment+(1|Month)+(1|Block),family=quasipoisson)
summary(m3)
Generalized linear mixed model fit by the Laplace approximation
Formula: invcount ~ Treatment + (1 | Month) + (1 | Block)
   AIC BIC logLik deviance
 43.54  62 -15.77    31.54
Random effects:
 Groups   Name        Variance  Std.Dev.
 Month    (Intercept) 0.0000000 0.000000
 Block    (Intercept) 0.0021038 0.045867
 Residual             0.0926225 0.304339
Number of obs: 160, groups: Month, 10; Block, 6

Fixed effects:
                   Estimate Std. Error t value
(Intercept)        -0.51644    0.05411  -9.545
Treatment2.Radiata -0.36246    0.08401  -4.314
Treatment3.Aldabra -0.29319    0.08197  -3.577

Correlation of Fixed Effects:
            (Intr) Trt2.R
Trtmnt2.Rdt -0.566
Trtmnt3.Ald -0.580  0.372

Log(Abundance) did not solve the problem of zero variance. If quasipoisson 
errors are not acceptable to use with abundance, i.e. non-integers, is 
there a family of errors that would be recommended? Or should I simply 
multiply abundance to obtain whole numbers?

Many thanks in advance,
Christine


--On 01 June 2009 23:17 -0400 Ben Bolker <bolker at ufl.edu> wrote:

> Emmanuel Charpentier wrote:
>> Le lundi 01 juin 2009 à 18:00 +0100, Christine Griffiths a écrit :
>>> Dear R users,
>>>
>>> I am having a problem with getting zero variance in my lmer models
>>> which  specify two random effects. Having scoured the help lists, I
>>> have read that  this could be because my variables are strongly
>>> correlated. However, when I  simplify my model I still encounter the
>>> same problem.
>>>
>>> My response variable is abundance which ranges from 0-0.14.
>>>
>>> Below is an example of my model:
>>>> m1<-lmer(Abundance~Treatment+(1|Month)+(1|Block),family=quasipoisson)
>>>> summary(m1)
>>> Generalized linear mixed model fit by the Laplace approximation
>>> Formula: Abundance ~ Treatment + (1 | Month) + (1 | Block)
>>>    AIC   BIC logLik deviance
>>>  17.55 36.00 -2.777    5.554
>>> Random effects:
>>>  Groups   Name        Variance   Std.Dev.
>>>  Month    (Intercept) 5.1704e-17 7.1906e-09
>>>  Block    (Intercept) 0.0000e+00 0.0000e+00
>>>  Residual             1.0695e-03 3.2704e-02
>>> Number of obs: 160, groups: Month, 10; Block, 6
>>>
>>> Fixed effects:
>>>                    Estimate Std. Error t value
>>> (Intercept)        -3.73144    0.02728 -136.80
>>> Treatment2.Radiata  0.58779    0.03521   16.69
>>> Treatment3.Aldabra  0.47269    0.03606   13.11
>>>
>>> Correlation of Fixed Effects:
>>>             (Intr) Trt2.R
>>> Trtmnt2.Rdt -0.775
>>> Trtmnt3.Ald -0.756  0.586
>>>
>>> 1. Is it wrong to treat this as count data?
>>
>> Hmmm... IST vaguely R that, when the world was young and I was (already)
>> silly, Poisson distribution used to be a *discrete* distribution. Of
>> course, this may or may not stand for "quasi"Poisson (for some value of
>> "quasi").
>>
>> May I inquire if you tried to analyze log(Abundance) (or log(Count),
>> maybe including log(area) in the model) ?
>>
>> HTH,
>>
>> 					Emmanuel Charpentier
>>
>>> 2. I would like to retain these as random factors given that I designed
>>> my  experiment as a randomised block design and repeated measures,
>>> albeit  non-orthogonal and unbalanced. Is it acceptable to retain these
>>> random  factors, is all else is correct?
>
>    I think so ...
>
>>> 3. The above response variable was calculated per m2 by dividing the
>>> Count  by the sample area. When I used the Count (range 0-9) as my
>>> response  variable, I get a small but reasonable variation of random
>>> effects. Could  anyone explain why this occurs and whether one response
>>> variable is better  than another?
>
>   To agree with what Emmanuel said above: you should use Count~...,
> offset=log(area) for the correct analysis ...  that should solve
> both your technical (zero random effects) and conceptual (even
> quasiPoisson should be discrete data) issues.
>
>>>
>>>> m2<-lmer(Count~Treatment+(1|Month)+(1|Block),family=quasipoisson)
>>>> summary(m2)
>>> Generalized linear mixed model fit by the Laplace approximation
>>> Formula: Count ~ Treatment + (1 | Month) + (1 | Block)
>>>    AIC BIC logLik deviance
>>>  312.5 331 -150.3    300.5
>>> Random effects:
>>>  Groups   Name        Variance Std.Dev.
>>>  Month    (Intercept) 0.14591  0.38198
>>>  Block    (Intercept) 0.58690  0.76609
>>>  Residual             2.79816  1.67277
>>> Number of obs: 160, groups: Month, 10; Block, 6
>>>
>>> Fixed effects:
>>>                    Estimate Std. Error t value
>>> (Intercept)          0.3098     0.3799  0.8155
>>> Treatment2.Radiata   0.5879     0.2299  2.5575
>>> Treatment3.Aldabra   0.5745     0.2382  2.4117
>>>
>>> Correlation of Fixed Effects:
>>>             (Intr) Trt2.R
>>> Trtmnt2.Rdt -0.347
>>> Trtmnt3.Ald -0.348  0.536
>>>
>>> Many thanks,
>>> Christine
>>>
>>
>> _______________________________________________
>> R-sig-mixed-models at r-project.org mailing list
>> https://stat.ethz.ch/mailman/listinfo/r-sig-mixed-models
>
>
> --
> Ben Bolker
> Associate professor, Biology Dep't, Univ. of Florida
> bolker at ufl.edu / www.zoology.ufl.edu/bolker
> GPG key: www.zoology.ufl.edu/bolker/benbolker-publickey.asc
>
> _______________________________________________
> R-sig-mixed-models at r-project.org mailing list
> https://stat.ethz.ch/mailman/listinfo/r-sig-mixed-models



----------------------
Christine Griffiths
School of Biological Sciences
University of Bristol
Woodland Road
Bristol BS8 1UG
Tel: 0117 9287593
Fax 0117 925 7374
Christine.Griffiths at bristol.ac.uk
http://www.bio.bris.ac.uk/research/mammal/tortoises.html




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