[R-meta] Question about a meta-analysis of 2 studies

Michael Dewey ||@t@ @end|ng |rom dewey@myzen@co@uk
Thu Nov 14 16:53:33 CET 2024


Dear Adelina

Comment in-line

On 14/11/2024 13:57, Adelina Artenie wrote:
> Hi Michael,
> 
> Thanks for the reply. In my code, I referenced the paper which 
> recommends this (counter-intuitive) approach: appendix 3 (I can’t seem 
> to be able to attach here).
> 
> There are different ways of implementing the same method. For example, 
> we could also do:
> 
> meta_inci <- metagen(TE = ln_inci,
> 
> lower = ln_LB,
> 
>                           upper = ln_UB,
> 
>                           studlab = idd_count,
> 
>                           data = df_inci,
> 
>                           sm = "IRLN",
> 
>                           method.tau ="SJ" ,
> 
>                           comb.fixed = FALSE,
> 
>                           comb.random = TRUE, backtransf = TRUE,
> 
>                             hakn = TRUE,
> 
>                           text.random = "Overall")
> 
> summary(meta_inci)
> 
> Both approaches produce the same results, so it does not seem to be a 
> coding problem.
> 
> Agree the variance is expected to be large but the estimated 95%CI are 
> unrealistic (0 - >1000).

No. It is perfectly realistic. It is not what you wanted but it reflects 
the lack of precision here.

Michael

> 
> Adelina
> 
> *From: *R-sig-meta-analysis <r-sig-meta-analysis-bounces using r-project.org> 
> on behalf of Michael Dewey via R-sig-meta-analysis 
> <r-sig-meta-analysis using r-project.org>
> *Date: *Thursday, 14 November 2024 at 13:42
> *To: *Adelina Artenie via R-sig-meta-analysis 
> <r-sig-meta-analysis using r-project.org>
> *Cc: *Michael Dewey <lists using dewey.myzen.co.uk>
> *Subject: *Re: [R-meta] Question about a meta-analysis of 2 studies
> 
> Dear Adelina
> 
> You state that you are interested in the HKSG method but I do not see an
> exampe of that in your code. You are also doing something which metafor
> regards as incompatible (knha with FE).
> 
> But the main problem is that you are trying to estimate a variance
> (tau^2) based on only two observations. This is in general very imprecise.
> 
> If you can clarify what your underlying scientific goal is it may be
> that somebody, quite likely not me< can offer a way forward.
> 
> Michael
> 
> 
> On 14/11/2024 11:11, Adelina Artenie via R-sig-meta-analysis wrote:
>> Hello,
>> 
>> The HKSG<https://bmcmedresmethodol.biomedcentral.com/articles/10.1186/1471-2288-14-25#MOESM1 <https://bmcmedresmethodol.biomedcentral.com/articles/10.1186/1471-2288-14-25#MOESM1>> approach has been proposed to be used when the number of studies to pool is small, instead of more traditional meta-analysis methods.
>> I have to pool several estimates in cases where there are only 2 estimates, often quite different from each other and with varying levels of precision.
>> In pretty much all cases, the HKSG method seems to break down, leading to unrealistic 95%CI (this seems to improve as soon as I have at least 3 estimates and gets better with more estimates).
>> Conceptually, I get it: we have only 2 studies and the estimates are very different, so a meta-analysis is not ideal. But if I still want to do it, do you know of other methods that could better account for heterogeneity than traditional methods, even if  imperfect?
>> I included some example code below.
>> Thanks
>> Adelina
>> 
>> 
>> library(meta)
>> library(metafor)
>> 
>> idd_count <- c(1, 2)
>> 
>> inci <- c(11.1849, 1.484536956)
>> CI95_LB <- c(6.8522, 1.042335486)
>> CI95_UB <- c(18.2571, 1.985159973)
>> df_inci <- data.frame(idd_count, inci, CI95_LB, CI95_UB)
>> 
>> # DL estimator for tau
>> df_inci$ln_inci <- log(df_inci$inci)
>> df_inci$ln_LB <-log(df_inci$CI95_LB)
>> df_inci$ln_UB <-log(df_inci$CI95_UB)
>> 
>> meta_inci <- metagen(TE = ln_inci,
>>                           lower = ln_LB,
>>                           upper = ln_UB,
>>                           studlab = idd_count,
>>                           data = df_inci,
>>                           sm = "IRLN",
>>                           method.tau = "DL", # switching between estimators (eg, REML, PM) gives the same results
>>                           comb.fixed = FALSE,
>>                           comb.random = TRUE, backtransf = TRUE,
>>                           text.random = "Overall")
>> summary(meta_inci)
>> 
>> 
>> # HKSJ approach: https://bmcmedresmethodol.biomedcentral.com/articles/10.1186/1471-2288-14-25#MOESM1 <https://bmcmedresmethodol.biomedcentral.com/articles/10.1186/1471-2288-14-25#MOESM1>
>> 
>> df_inci$ln_SE <- (df_inci$ln_inci - df_inci$ln_LB) /  1.96
>> 
>> meta_modellll <- rma.uni(yi = ln_inci,
>>                           sei = ln_SE,
>>                           method = "FE",  # intentionally set as FE, following recommendations by Inthout et al 2014
>>                           knha=TRUE,
>>                           data = df_inci)
>> summary(meta_modellll)
>> 
>> point_estimate <- exp(meta_modellll$b)
>> lower_bound <- exp(meta_modellll$ci.lb)
>> upper_bound <- exp(meta_modellll$ci.ub)
>> cat("Point Estimate:", point_estimate, "\n")
>> cat("95% CI Lower Bound:", lower_bound, "\n")
>> cat("95% CI Upper Bound:", upper_bound, "\n")
>> 
>> 
>> 
>> 
>> 
>> 
>> 
>> 
>> 
>>        [[alternative HTML version deleted]]
>> 
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> 
> -- 
> Michael
> 
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