ID

a_t1    a_t2    b_t1    b_t2
CACCCGTAGAACCGACCTTGCG_mmu-miR-99b-5p    1578    1941    2348    10941
CACCCGTAGAACCGACCTTGC_mmu-miR-99b-5p    442    426    564    3839
CACCCGTAGAACCGACCTTG_mmu-miR-99b-5p    54    43    66    253
CCGTAGAACCGACCTTGCG_mmu-miR-99b-5p    26    33    33    157
CGTAGAACCGACCTTGCG_mmu-miR-99b-5p    23    22    38    187
CACCCGTAGAACCGACCT_mmu-miR-99b-5p    17    27    31    189
ACCCGTAGAACCGACCTTGCG_mmu-miR-99b-5p    14    19    30    94

I want to run an ANOVA test on the rows above. I have two conditions with
two replicates each. I assume that there is no difference between a and b
for the different rows. I want to get which rows a different between a and
b.
How do I approach this in R?

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