Hi All,

I have a microarray dataset as follows:

                          expt1 expt2 expt3  expt4 expt 5 

gene1                val      val     val      val     val

gene2                val      val     val      val    val

.
.
..
gene15000       val       val     val      val     val


The result is from the same organism in four different experiments.  Also, there are 4 replicates of each experiment. My aim was to find genes that are statistically significant across the four experiments. I carried out one-way anova as follows:


sTest<-read.table("myData.dat",header = T, row.names = 1)
group <- gl(4,4,16, label=c("Glucose","Citrate", "Tide","Dawn"))

gm1 <- (x~group, data=sTest)

I received error messages that "x" is unknown, and did not know how to go further from here.

Please help!!!


James

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