Hello,

I have RNAseq data, which I am trying to analyze with DESeq. My file (tab delimited .txt) appears to be correct:

>head(myfile)
    	VZ_w13 VZ_w14a VZ_w14b VZ_w15a VZ_w15b VZ_w16a
	ENSG00000253101      0       0       0       0       0       0
	ENSG00000223972      0       0       0       0       0       0...

However, when I try to analyze the data with

>cds <- newCountDataSet(myfile,conds)

I get the following message:

"Error in newCountDataSet(myfile,conds) : The countData is not integer.

The problem, as far as I can tell, is that my data are numerical, not integer, because when I run

>str(myfile)
	'data.frame':	53433 obs. of  14 variables:
	 $ VZ_w13   : num  0 0 0 0 8 0 0 0 0 0 ...

Does anyone have a way to convert my file from numerical to integer? As you can see, the data are in fact integers are already, so I'm a bit confused. 

Thanks,
Eric


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