Hi guys
My data is Tasmania txt
There are *N *= 16 samples, consisting of 8 replicate  cores (taken from
different areas across the  sandflat) from each of 2 natural
'treatments'  (either
disturbed "D" or undisturbed "U" by soldier crab burrowing activity. The
abundances of each of *p *= 56 species were recorded from each core
(variables 1 to 39 in the file are  nematode worms and variables 40-56 in
the file are copepods, as identified by the column  headed "Taxon"). Note
that the *rows *are the species and the *columns *are the scores (sample
units) in the raw data file.
When i want to do ANOSIM i get an NaN error message. What is wrong?
R output is here under
> tasmania.df=read.table(file.choose(),header=T)
> attach=(tasmania.df)
> tasmania.df
                Species Taxon D1      D2  D3 D4     D5      D6 D7 D8  U1
U2      U3 U4 U5 U6  U7     U8
1         Actinonema.sp   Nem  0   0.000   0  0  0.000   0.000  0  0   0
2.48   0.000  2  1  0   0  0.000
2        Axonolaimus.sp   Nem 10   8.995  12 14  6.325   0.000  0  4  51
48.36  49.237 26  9  5  37 50.550
3        Bathylaimus.sp   Nem  0   0.000   0  1 72.105  42.570 15 16   1
0.00   6.005  0 51  8  21 11.795
4       Calyptronema.sp   Nem  0   0.000   0  0  0.000   0.000  0  0   0
0.00   0.000  0  1  0   1  1.685
5         Chaetonema.sp   Nem  0   0.000   0  0 13.915   7.095  0  2   0
0.00   0.000  0  1  0   0  1.685
6      Chromaspirina.sp   Nem  0   0.000   0  0  0.000   0.000  0  0   0
0.00   1.201  1  0  0   0  0.000
7           Comesoma.sp   Nem  0   0.000   0  0  0.000   0.000  3  0   0
0.00   4.804  0  4  2   0  0.000
8          Daptonema.sp   Nem  0   0.000   0  3  1.265   9.460  3  1   0
9.92   6.005  6  5  0   3  5.055
9        Desmodora.sp.A   Nem  0   0.000   0  0  0.000   0.000  0  1   2
2.48   0.000  0  1  0   0  0.000
10       Desmodora.sp.B   Nem  0   0.000   0  0  0.000   0.000  0  0   0
0.00   8.406  2  4  2   0  3.370
11        Enoploides.sp   Nem  0   0.000   0  0  1.265   0.000  0  0   0
0.00   0.000  0  0  0   0  1.685
12           Enoplus.sp   Nem  0   0.000   0  0  0.000   0.000  0  2   0
0.00   0.000  0  0  0   0  0.000
13     Epacanthion.sp.A   Nem  1  10.280   1  5  1.265   0.000  3  1   0
3.72   1.201  1  1  0   5 13.480
14     Epacanthion.sp.B   Nem  3   3.855   0  0  0.000   0.000  1  4  16
14.88   3.603  0  0  0   1  1.685
15      Eubostrichus.sp   Nem  0   0.000   0  0  0.000   0.000  1  0   0
0.00   1.201  0  0  0   0  1.685
16       Eurystomina.sp   Nem  1   0.000   0  0  1.265   0.000  1  1   1
3.72   0.000  1  0  0   1  0.000
17 Hypodontolaimus.sp.A   Nem 45 201.745 106 69 78.430 319.275 50 87  18
12.40 109.283 92  6  6   9 23.590
18 Hypodontolaimus.sp.B   Nem  0   0.000   0  0  1.265   0.000  0  0  51
85.56  15.612 14  2  1   2  5.055
19       Leptolaimus.sp   Nem  0   0.000   0  0  0.000   0.000  0  0   0
0.00   0.000  0  1  0   0  0.000
20      Leptonemella.sp   Nem  0   0.000   0  0  0.000   0.000  0  1   1
1.24   3.603  0  1  1   0  0.000
21      Mesacanthion.sp   Nem 14  15.990  18  8  2.530   7.095  0  3  30
28.52   8.406  6  1  1   5 15.165
22       Microlaimus.sp   Nem  0   0.000   0  0  0.000   0.000  0  0   0
0.00   0.000  0  0  2   2 26.960
23        Monhystera.sp   Nem  0   0.000   1  1  0.000   0.000  3  0   0
0.00   0.000  0  0  0   0  0.000
24  Nannolaimoides.sp.A   Nem  0   0.000   2  1  1.265   2.365  0  1   7
1.24   3.603  3  1  3   7  8.425
25  Nannolaimoides.sp.B   Nem  0   0.000   0  0  0.000   0.000  1  2   0
0.00   0.000  0  0  0   1  1.685
26   Neochromadora.sp.A   Nem  1   5.140  10  6 10.120   0.000  7 18   7
2.48  13.210  4  2  1   9 20.220
27   Neochromadora.sp.B   Nem  0   2.570   0  0  0.000   2.365  0  0   1
0.00   0.000  1  1  0   0  0.000
28       Odontophora.sp   Nem  1   0.000   0  0  1.265   0.000  1  0   0
0.00   1.201  0 28  1   4  1.685
29       Oncholaimus.sp   Nem  1   1.285   4  2 16.445  14.190 16 17   1
7.44   7.205  6 11  4  25 50.550
30              Onyx.sp   Nem  0   0.000   0  0 29.095  37.840 12 15   3
1.24   8.406  1  1  4  49 85.935
31   Paracanthonchus.sp   Nem  0   1.285   1  2  1.265   0.000  1  0   0
8.68   6.005  7  1  1   3  0.000
32         Polysigma.sp   Nem  0   0.000   1  0  0.000   0.000  0  0   0
0.00   0.000  0  0  0   0  0.000
33  Praeacanthonchus.sp   Nem  0   1.285   0  0  1.265   0.000  0  6   2
0.00   6.005  0  5  4   8  5.055
34     Promonhystera.sp   Nem  4   1.285   6 12  6.325  18.920  1  0   0
1.24   2.402  5  1  0   0  0.000
35   Pseudosteineria.sp   Nem  0   0.000   0  2  0.000   0.000  0  0   0
0.00   2.402  0  0  0   0  0.000
36        Sabatieria.sp   Nem  1   0.000   0  0  3.795  11.825  3  0   0
0.00   0.000  0 12  1   2  0.000
37     Spilophorella.sp   Nem  0   0.000   0  0  0.000   0.000  0  0   0
0.00   0.000  0  1  0   0  0.000
38     Symplocostoma.sp   Nem  0   0.000   0  0  0.000   0.000  0  2   2
3.72   0.000  0  0  0   0  0.000
39          Viscosia.sp   Nem  0   1.285   0  0  2.530   0.000  0  1   2
8.68   0.000  1  0  0   3  0.000
40            Ameira.sp   Cop 43  63.000   4  5  7.000   6.000 69  5 124
105.00  91.000 57 10 60 142 96.000
41      Apodopsyllus.sp   Cop  0   0.000   0  0  0.000   0.000  4  1   0
0.00   0.000  0  0  1   3  2.000
42        Ectinosoma.sp   Cop  0   0.000   0  0  0.000   0.000  1  0   0
2.00   0.000  0  1  4   6  7.000
43   Ectinosomatidae.sp   Cop  1  15.000  14  4  2.000   3.000  1  1   7
7.00   4.000  5  5  0   2  1.000
44     Haloshizopera.sp   Cop  0   0.000   0  0  0.000   0.000  0  0   2
0.00   0.000  0  0  0   0  0.000
45     Leptastacus.sp.A   Cop 30  97.000  27 35  3.000   1.000 29 47 151
117.00  15.000 88  3  0   6  2.000
46     Leptastacus.sp.B   Cop  1  11.000   3  0  0.000   0.000  0  1   0
0.00   2.000  5  0  0   0  0.000
47     Leptastacus.sp.C   Cop  0   0.000   0  0 10.000 180.000  3  1   0
0.00   0.000  0  0 10   0  0.000
48    Mictyricolatypica   Cop  0   0.000   8  3  0.000   1.000  3  5   0
0.00   0.000  0  1  0   0  0.000
49       Parevansula.sp   Cop  0   0.000   2  0  0.000   0.000  0  0   0
1.00   0.000  1  0  0   0  0.000
50  Quinquelaophonte.sp   Cop  0   0.000   0  0  0.000   0.000  0  0   2
3.00   0.000  0  0  0   2  0.000
51        Rhizothrix.sp   Cop  1   0.000   0  0  0.000   0.000  0  0   6
6.00   0.000  0  0  0   0  0.000
52       Undetermined.A   Cop  0   0.000   0  0  0.000   0.000  0  0   0
1.00   0.000  0  0  0   0  0.000
53       Undetermined.B   Cop  1   0.000   0  0  0.000   0.000  0  0   0
0.00   0.000  0  0  0   0  0.000
54       Undetermined.C   Cop  0   0.000   0  0  0.000   0.000  0  0   0
0.00   0.000  0  1  0   0  0.000
55       Undetermined.D   Cop  0   0.000   0  0  0.000   0.000  0  0   0
0.00   0.000  0  0  0   0  1.000
56       Undetermined.E   Cop  0   0.000   0  0  0.000   0.000  0  0   0
0.00   0.000  0  0  0   0  1.000
> Y=t(tasmania.df[,3:18])
> Y.t=Y^(0.5)
>  library(MASS)
>   my.dist = function (Y,measure="Euc") {
+    N=dim(Y)[1]; p=dim(Y)[2]
+    d=matrix(0,nrow=N,ncol=N)
+    for(i in 1:(N-1)) {
+       for(j in (i+1):N) {
+          d[i,j] = switch(measure,
+    SM = 1.0-(sum((Y[i,]>0)&(Y[j,]>0))+sum((Y[i,]==0)&(Y[j,]==0)))/p,
+    Jac = 1.0-sum((Y[i,]>0)&(Y[j,]>0))/sum((Y[i,]>0)|(Y[j,]>0)),
+    Euc = sqrt( sum((Y[i,]-Y[j,])^2) ),
+    BC=(sum(abs(Y[i,]-Y[j,]))/sum(Y[i,]+Y[j,])),
+    Man=sum(abs(Y[i,]-Y[j,])),
+    Manx= sum(abs(Y[i,]-Y[j,]))/length(Y[i,][Y[i,]+Y[j,]>0]),
+    Chi=sqrt(sum( (sum(Y)/apply(Y,2,sum))*
+                  ( Y[i,]/sum(Y[i,]) - Y[j,]/sum(Y[j,]) )^2))
+          )}
+       }
+    d = d+t(d)
+    return(as.dist(d))
+    }
> D=as.matrix( my.dist(Y.t,measure="BC") )
> tasmania.df=my.dist(Y.t,measure="BC")
> tasmania.mds=isoMDS(tasmania.df, k=2)
initial  value 15.803771
iter   5 value 11.418948
iter  10 value 10.784872
final  value 10.663487
converged
> plot(tasmania.mds$points, type = "n", axes=FALSE,
+      xlab="",ylab="", main="MDS of Tasmania data")
> text(tasmania.mds$points, labels =  rownames(Y))
> box()
> tasmania.mds$stress
[1] 10.66349
> tasmania.stress=round(tasmania.mds$stress/100,digits=3)
> mtext(paste("Stress = ",tasmania.stress,sep=""))
> group=rownames(Y)
> N=length(group)
> d.vec=rep(0,(N*(N-1)/2) )
> iwithin=rep(0,(N*(N-1)/2) )
> icount=0
>    for(i in 1:(N-1)) {
+       for(j in (i+1):N) {
+          icount=icount+1
+          d.vec[icount]=D[i,j]
+          if(group[i]==group[j]) iwithin[icount]=1
+          }
+       }
> r=rank(d.vec)
> r
  [1]   8  15  16  74 116  36  60  59  64  44  19 109  96  83  95   7  20
79 103  63  50  32  41  26   5 113 114  86  93   2  58  97  68
 [34]  33  89  82  35  10 104 118 108 112  42  88  37  31  87  76  28  11
84 117  85 102  14  23   9 107 106  40  53  30  65  22  57  77
 [67]  71 120 119  92  99  78 115 101 105  13  98  81  25  56  66  49  18
48  67  80  29  47  70  91  43  54   1  27  21 111 100  38  62
[100]  24   6  94 110  39  69   4  51  52  17  12  75  90  55  73  45  46
72  34  61   3
> r.w=sum(r*iwithin)/sum(iwithin)
> r.w
[1] NaN


Thanks

Uma

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