Hi, 

I would be extremely grateful if somebody can help with following
problem.

 We have done an experiment using a reference design with technical and
biological replicates where the target file looks like this:

FileName          Cy3      Cy5

xxx                   A          REF

xxx                   A          REF

xxx                   A          REF

xxx                   B          REF

xxx                   B          REF

xxx                   B          REF

xxx                   C          REF

xxx                   C          REF

xxx                   C          REF

xxx                   D          REF

xxx                   D          REF

xxx                   D          REF

 

Where A and B and C and D are biological replicates. Goal is now to find
differential expressed genes between AB and CD.

This is the script I used already but I doubt it very much because I
have results with exactly the same adjusted p Value.

Thanks for any help

Martin

 

 

Dipl.Ing(FH) Martin Radolf

Microarray Facility/Biooptics Group

Research Institute for Molecular Pathology (IMP)

Dr.Bohrgase 7

A-1030 Vienna

*.: 0043-(01)-79730-3703

*: radolf@imp.univie.ac.at

Web.: http://www.imp.ac.at/research/biooptics/

 

 

 

 


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