
Hi,

I'm trying to use the i-best software. Does anyone have experience from this, I can't get it to work with my data.

Here is the code: 

T1 <- read.delim(file="S://SEDIM//Yvonne//2_5//T1.txt",col.names= c("Dye/Sample_Peak", "Sample_File_Name", "Size", "Height", "Area_in_Point", "Area_in_BP", "Data_Point", "Begin_Point",    "Begin_BP", "End_Point", "End_BP", "Width_in_Point", "Width_in_BP", "User_Comments", "User_Edit"))
T1 <- subset(T1, Size < 1000 & Size > 50)
T1.B <- cbind(T1[grep("^B", as.character(T1$Color),perl=T),3],T1[grep("^B", as.character(T1$Color),perl=T),5])
T1.B <- cbind(T1.B, T1.B[,2]/sum(T1.B[,2]))


It works alright until the last two lines.  I try to grep the columns 3 and 5, but the outcome is 
 T1.B
     [,1] [,2]. 
I don´t quite understand the code of as.character(t1$Color), perl=T. Could anyone please explane what this does, I havn´t found it anyware and I am so stuck. Maybe I'm doing something else wrong as well..

I would really appreciate some help, thank you.

Yvonne


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