[R] Differential Gene Expression in R

Anas Jamshed @n@@j@m@hed1994 @end|ng |rom gm@||@com
Sun Aug 22 20:13:34 CEST 2021


 I have downloaded data from:
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE162562&fbclid=IwAR0iZQhttG8HzGhFIIMWbFgNszQrVDgiyVChYzQ_ypCx_d-1pn_tm7STjGs

and now I want to compare:
healthy vs Mild healthy vs Highly exposed seronegative (ishgl) Healthy vs
Asymptomatic covid19 patient healthy vs Highly exposed seronegative (non
ishgl)  from this data.

I started  like  :

library(edgeR)
library(limma)
library(GEOquery)
library(Biobase)

Sys.setenv("VROOM_CONNECTION_SIZE" = 131072 * 2)

setwd("D:\\")

untar("GSE162562_RAW.tar")

filelist = list.files(pattern = ".*.txt.gz")



But after getting text files I don't know how to proceed further. I want to
find degs from these files  *Plz help me *

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