[R] Plotting DMRs (Differentially Methylated Regions) using Gviz package in R

pooja sinha pj@|nh@07 @end|ng |rom gm@||@com
Fri Feb 7 19:52:07 CET 2020


Thanks, I'll check it out.

On Fri, Feb 7, 2020 at 1:08 PM Martin Morgan <mtmorgan.bioc using gmail.com>
wrote:

> Probably have more success asking on https://support.bioconductor.org.
>
> Martin Morgan
>
> On 2/7/20, 12:57 PM, "R-help on behalf of pooja sinha" <
> r-help-bounces using r-project.org on behalf of pjsinha07 using gmail.com> wrote:
>
>     Hi All,
>
>     I have a file list consisting of Chromosome, Start , End & Methylation
>     Difference in the following format in excel:
>
>     Chrom     Start          End          Meth. Diff
>
>     chr1     38565900 38566000 -0.20276818
>
>     chr1     38870400 38870500 -0.342342342
>
>     chr1     39469400 39469500 -0.250260552
>
>     chr1     52013600 52013700 -0.37797619
>
>     chr1     52751700 52751800  0.257575758
>
>     chr1     75505100 75505200 -0.262847308
>
>     I need help in plotting the DMRs using Gviz package in R. I tried a
> code
>     below but it doesn't turn out correct.
>
>     library(GenomicRanges)
>     library(grid)
>     library(Gviz)
>     library(rtracklayer)
>     library(BSgenome)
>     library(readxl)
>     library(BSgenome.Rnorvegicus.UCSC.rn6)
>     genome <- getBSgenome("BSgenome.Rnorvegicus.UCSC.rn6")
>     genome
>     data1 <- read_excel("DMRs_plots.xlsx")
>     head(data1)
>     data1$Chrom = Chrom$chr1
>
>     track1 <- DataTrack(data = data1, from = "38565900" , to = "282250000",
>     chromosome = Chrom$chr1, name = "DMRs")
>
>     itrack <- IdeogramTrack(genome = genome, chromosome = chr)
>
>     plotTracks(track1, itrack)
>
>
>     If anyone know how to plot and correct my code including how to add
>     methylation difference values, then that will be of great help.
>
>
>     Thanks,
>
>     Puja
>
>         [[alternative HTML version deleted]]
>
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