[R] particle count probability

[PIKAL Petr]

Dear all

Sorry, this is probably the most off-topic mail I have ever sent to this help list. However maybe somebody could point me to right direction or give some advice.

In microscopy particle counting you have finite viewing field and some particles could be partly outside of this field. My problem/question is:

Do bigger particles have also bigger probability that they will be partly outside this viewing field than smaller ones?

Saying it differently, although there is equal count of bigger (white) and smaller (black) particles in enclosed picture (8), due to the fact that more bigger particles are on the edge I count more small particles (6) than big (4).

Is it possible to evaluate this feature exactly i.e. calculate some bias towards smaller particles based on particle size distribution, mean particle size and/or image magnification?

Best regards
Petr Pikal
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