Hi, I am trying to read a fasta file with >15K alignedd sequences. However it crashes showing a runtime error. I have tried both R.3.2.3 and R.3.2.1 under windows 7. A smaller fasta file works perfectly fine in either case. library(seqinr) myseqs <- read.alignment("aligned_seqs.fasta",format="fasta") Thanks, Eric [[alternative HTML version deleted]]