[R] ShortRead with BWA
Duke
duke.lists at gmx.com
Wed Apr 28 00:07:43 CEST 2010
Dear folks,
Please welcome a newbie both to R and the mailing list :). I am
currently working on a sequencing project, and heard about R as well as
some of its packages for next gen sequencing, and decided to give it a
try. Starting with ShortRead, I found a document
(http://www.bioconductor.org/packages/2.5/bioc/vignettes/ShortRead/inst/doc/ShortRead_and_HilbertVis.pdf)
which does mention that readAligned was extended to support SAM format
(for example, BWA output).
So I tried readAligned with
BWAAln <- readAligned(BWAFile, type = "BWA")
but then I got error:
> BWAAln <- readAligned(BWAFile, type = "BWA")
Error: UserArgumentMismatch
arugment 'type' had value 'BWA' allowable values: 'SolexaExport'
'SolexaAlign' 'SolexaPrealign' 'SolexaRealign' 'SolexaResult'
'MAQMap' 'MAQMapShort' 'MAQMapview' 'Bowtie' 'SOAP'
It looks like ShortRead does not support BWA. Am I right, or what am I
doing wrong? Anybody has any idea?
Thanks,
D.
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