[R] microarray analysis in R without replicates

johannes rara johannesraja at gmail.com
Tue Sep 22 20:37:14 CEST 2009


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-Johannes

2009/9/22 Rainer Tischler <rainer_t62 at yahoo.de>:
> Dear all,
>
> I have received a microarray data set in standard Affymetrix CEL-format consisting of only six samples  without any replicates (same organism and cell type, but different individuals and different biological conditions for each individual; the same Affymetrix GeneChip platform was used for all samples). Moreover, the data was apparently collected without any a-priori biological hypothesis.
>
> I know that it is impossible to apply standard clustering, feature selection or classification techniques in this case. However, I am wondering whether anybody is aware of a method in R to extract meaningful biological information in this case (i.e. from single-sample microarray data or from multiple samples with different biological conditions and no replicates) - or is there nothing I can do given the above limitations?
>
> Many thanks,
> Rainer
>
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