[R] Lag correlation

[gauravbhatti]

Hi
I have a data frame which looks like :
      hs361224   hs426435   hs903411   hs205893  sPP13_2  hs792300  hs747811
2.5  0.4444837 -0.7684995 -0.4209555 -0.4996375 1.363161 -0.259850 -0.503650
5     1.8617705 -0.5842845 -0.3615625 -0.9784640 1.862586  2.104920 
1.192305
24    
......................................................................................................
.
.
96      
...............................................................................................

where each column represents a gene and row names are time steps.My goal is
to calculate the correlations between different columns (genes) at lag
values of +1 and -1.
 
I am trying to use the R function acf with lag.max = 1. But I am not
confident about the results since I am not sure how the function works. For
example in some cases it uses lag value of 1 and in others it uses -1. Is
that a random choice or is there a procedure it follows? 
Is there a better way calculate lag correlations or if this is the best way
then can someone explain how the acf fn actually works?  

Gaurav Bhatti
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