[R] A question about r anaysing microarry data!Thank you!
Li, Bo
bo.li at ttu.edu
Thu Jun 25 23:14:55 CEST 2009
Dear,
Thank you for cheking the mail!
I'm Bo, student in TTU, and had a problem in using r for analyzing microarry data.
This is what I write in r:
library(limma)
argets <- readTargets(file="targets.txt") *targets.txt is a tab-delimited file with columns: "SlideNumber", "FileName", "Cy3", "Cy5". The filenames refer to the feature extraction files for each array in the experiment.
RG<-read.maimages(targets, source="agilent",wt.fun=myfunFE,columns=list(R="rProcessedSignal",G="gProcessedSignal"))
*In this step , cannot open file'Imm 10 Cy3_TM 10 Cy5 ': No such file or directory
,and I 've tried to path= "d:\\Imm 10 Cy3_TM 10 Cy5" or setwd ,but the same error, so I donot know how to tell to find this file,could you help me to figure it out?
Thank you so much!
Sincerely,
Bo
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