[R] Re al Time PCR data analysis help

[gauravbhatti]

I have looked at the package .........it won't work for me since it requies
us to read files with the format :
The text file must have the following structure: 
      1st column = names denoting genes or primer pairs 
      2nd column = plate index of each gene or primer pair 
      remaining columns = (replicate) Ct values. 
In my case the data I have is like following:
Well	Sample Name	Detector	Task	Ct	StdDev Ct	Qty	Mean Qty	StdDev Qty	Filtered
Tm	User Defined #1	User Defined #2	User Defined #3
A1	4490	ADORA2A	Unknown	27.5961	0.236								
A2	4490	ADORA2A	Unknown	27.1953	0.236								
A3	4490	ADORA2A	Unknown	27.6102	0.236								
A4	5775	ADORA2A	Unknown	31.3386	0.296								




Andrej Kastrin-3 wrote:
> 
> Check out qpcR package.
> 
> Andrej
> 
> gauravbhatti wrote:
>> Hi I need help since I have never worked on RT PCR data before .  I have
>> 8
>> data files each corresponding  to a single pcr run. Each file also
>> represent
>> 10 samples(5 cancer and 5 healthy) belonging to a unique gene. I have 7
>> unique genes and 1 reference (total 8 files). Can any body help me with
>> preproceesing and normalization?
> 
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> 

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