[R] creating standard curves for ELISA analysis

1Rnwb sbpurohit at gmail.com
Tue Dec 9 16:44:10 CET 2008


Hello R guru's

I am a newbie to R, In my research work I usually generate a lot of ELISA
data in form of absorbance values. I ususally use Excel to calculate the
concentrations of unknown, but it is too tedious and manual especially when
I have 100's of files to process. I would appreciate some help  in creating
a R script to do this with minimal manual input. s A1-G1 and A2-G2 are
standards serially diluted H1 and H2 are Blanks. A3 to H12 are serum
samples. I am pasting the structure of my data below:



A1		14821
B1		11577
C1		5781
D1		2580
E1		902
F1		264
G1		98
H1		4
A2		14569.5
B2		11060
C2		5612
D2		2535
E2		872
F2		285
G2		85
H2		3
A3		1016
B3		2951.5
C3		547
D3		1145
E3		4393
F3		4694
G3		1126
H3		1278
A4		974.5
B4		3112.5
C4		696.5
D4		2664.5
E4		184.5
F4		1908
G4		108.5
H4		1511
A5		463.5
B5		1365
C5		816
D5		806
E5		1341
F5		1157
G5		542.5
H5		749

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