Hi Dr. Anders, I would just like to consult about our Illumina run. We have
two groups of samples from a non-model organism. Group A organisms are
considerably larger than Group B, which are as small as a dot. As a
result,  one QIAgen RNeasy extraction from from Group B requires a larger
number of samples. For each group of samples we have 4 libraries from 4
corresponding extractions. Thus, we have a total of 8 libraries. All
libraries from Group A have n=7. On the other hand, all libraries from
Group B have n=21. Given the unequal sample size from each library, I would
like to ask if differential expression analysis between Groups A and B will
still be valid?

Thanks,
G

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