Hi Camille

There are some examples in the vignette for the package.

You can see an example file by using:

path <- system.file("exData", package = "ReadqPCR")
qPCR.example <- file.path(path, "qPCR.example.txt")

More details are given in section 3, page 7 of the vignette, of the
different columns needed.


It will work without technical replicates, as long as the gene names
are distinct. Presumably you have biological replicates?


Jim







On 14 June 2014 14:56, Camille [guest] <guest@bioconductor.org> wrote:

> Dear all,
>
> I'm a phD student in Center of Cancerology of Toulouse France. I'm a new R
> user, and I would like to use the package "NormqPCR" for the functions
> geNorm and Normfinder.
> I don't have raw data file, I have a file which I can modify
> with sample names, gene names and Ct values where I don't have replicates
> because I was using Fluidigm method for amplification.
> How to do to use the function read.qPCR, how to configure my tab with Ct
> values to allow R to load my data?
> Best regards
>
> Camille
>
>  -- output of sessionInfo():
>
> read.qPCR
>
> --
> Sent via the guest posting facility at bioconductor.org.
>
>


-- 
James R Perkins PhD
Marie Curie Post-Doctoral Researcher
Research Laboratory, Allergy Unit
University Hospital of Malaga-IBIMA
29009 Malaga, Spain

	[[alternative HTML version deleted]]

