Manoeli
When you say you have decimals are they numbers between 0 and 1 or are they
larger?

You can see how to use DESeq2 on count data, which could be approprriate
here as you have different
library sizes in the supplementary material of the paper by PJ McMurdie
http://www.ploscompbiol.org/article/info%3Adoi%2F10.1371%2Fjournal.pcbi.1003531
 and I here:
http://joey711.github.io/waste-not-supplemental/

Best
Susan

Susan Holmes
Professor, Statistics and BioX
John Henry Samter University Fellow
in Undergraduate Education
Director, Mathematical and Computational Sciences
Stanford
http://www-stat.stanford.edu/~susan/



On Thu, Apr 24, 2014 at 5:13 PM, Manoeli Lupatini <mlupatini@gmail.com>wrote:

> Hi,
>
>
> I have counts of DNA for 16S with different library sizes and want to use
> DESeq2 to normalize the counts. However, I used Picrust to correct the 16S
> copy number for OTUs and the number generated by this correction are not
> integers (but decimals). Can I used DESeq2 to normalized my count data
> (using size factor) obtained by this 16S number correction considering that
> the DESeq2 was developed based in counts and not in counts corrected by 16S
> copy number?
>
>
> Thanks,
>
>
> Manoeli
>
> --
>
> Manoeli Lupatini
> PhD candidate
> Netherlands Institute of Ecology (NIOO/KNAW)
> Wageningen, The Netherlands
>
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