Dear Paolo,

sounds like you want:
(D - C) - (B - A)

Cheers,

 - axel



On Tue, Apr 15, 2014 at 9:16 AM, Paolo Kunderfranco <
paolo.kunderfranco@gmail.com> wrote:

> Dear All,
>
> Could you please help me to design a contrast for Differential Analysis?
>
> ....
> constrast.matrix <- makeContrasts (
> --------,
> levels=design)
> ...
>
>
> I have 4 groups.
>
> A - normal
> B - cancer
> C - normal + drug
> D - cancer + drug
>
> I would like to find genes modulated in D versus C. These genes should be
> not affected or change significantly in B versus A
>
>
> Any suggestion?
>
> Many thanks,
>
> Paolo
>
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>
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