HI,

Object : problem with hgu133plus2.db  you need to install this package to complete this step

If possible can anyone help. I am new to R and are working through the tutorial of Daniel Swan (Analysing microarray data in BioConductor)
Upon writing the following command I get the following error:

> biocLite("hgu133plus2.db")
BioC_mirror: http://bioconductor.org
Using Bioconductor version 2.13 (BiocInstaller 1.12.0), R version 3.0.3.
Installing package(s) 'hgu133plus2.db'
Warning: package 'hgu133plus2.db' is in use and will not be installed
Warning message:
installed directory not writable, cannot update packages 'boot', 'class',
  'cluster', 'foreign', 'KernSmooth', 'MASS', 'Matrix', 'mgcv', 'nlme', 'nnet',
  'rpart', 'spatial'


Thus, needless to say when I continue with the next command I get more errors:

> library(hgu133plus2.db)
> library(annotate)
> gene.symbols <- getSYMBOL(probeset.list$ID, "hgu133plus2")
Error in unlist(lookUp(x, data, "SYMBOL")) :
  error in evaluating the argument 'x' in selecting a method for function 'unlist': Error in lookUp(x, data, "SYMBOL") : No keys provided


Can anyone help please
Regards
Rabia


_______________________________________
Rabia Johnson (PhD)
Senior Scientist
South African Medical Research Council
Diabetes Discovery Platform
Building E
Francie van Zijl Drive, Parow Valley, 7505, Cape Town
Cell: +27 82 0677296
Tel: +27 21 938 0866
Fax: +27 21 938 0456
E-mail: rabia.johnson@mrc.ac.za


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