Hi Jerry,

Batch information is often annotated in a data set. If it is not, one way
to annotate batches is to identify what time each sample was run and then
see if they cluster into distinct groups - which you could call batches.
Finally, the surrogate variable analysis approach with the sva() function
takes as input the data matrix (normalized) and the corresponding
information about the primary variables you care about and attempts to
recover the batches from the microarray data themselves.

I hope that helps.

Jeff


On Mon, Mar 17, 2014 at 9:00 PM, Jerry Cholo <jerrycholo@gmail.com> wrote:

> Hello,
>
> I would like to remove the batch effects from a gene expression data using
> Surrogate Variable Analysis (SVA).  When I looked at the SVA (
> http://www.bioconductor.org/packages/release/bioc/html/sva.html) and
> "bladderbatch", I noticed that for 57 different samples, there are 5
> different batches.  May someone let me know how I could define these
> batches for my own data?  In fact, my datasets include the normal, disease,
> two different tissues, and two different chip arrays?
>
> Thanks,
>
> Jerry
>
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