Dear all,

I'm having trouble reading FCS files created with BD FACSCalibur, CellQuest
5.2.1. The files can be opened with FlowJo and after exporting from FlowJo
into FCS2 they can also be opened in R. However, I'd like to be able to
load all of them with flowCore at once.

Are there any known issues with this? Is there a workaround how to read
them directly with flowCore?

Best wishes
Anja

sessionInfo()

R version 3.0.2 (2013-09-25)
Platform: x86_64-w64-mingw32/x64 (64-bit)

locale:
[1] LC_COLLATE=German_Germany.1252  LC_CTYPE=German_Germany.1252
 LC_MONETARY=German_Germany.1252
[4] LC_NUMERIC=C                    LC_TIME=German_Germany.1252

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base

other attached packages:
[1] flowCore_1.28.0       BiocInstaller_1.12.0  ProjectTemplate_0.4-2
testthat_0.7.1

loaded via a namespace (and not attached):
 [1] Biobase_2.22.0     BiocGenerics_0.8.0 evaluate_0.5.1     feature_1.2.9
     graph_1.40.0
 [6] ks_1.8.13          MASS_7.3-29        mvtnorm_0.9-9996
parallel_3.0.2     pcaPP_1.9-49
[11] robustbase_0.9-10  rrcov_1.3-4        stats4_3.0.2       stringr_0.6.2
     tools_3.0.2


-- 
Dr. Anja Mirenska

Team Chipcytometry
Dept. Paed. Pneumology, Allergology and Neonatology
I10 E02 R7370
Hannover Medical School
Carl-Neuberg-Strasse 1
30625 Hannover
GERMANY

phone: +49-511-5327826
 web: www.chipcytometry.com

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