Hi



I am analyzing  microarray data *of Plasmodium falciparum* parasite , un
like  the normal CGH, we have CY3 labeled complicated malaria  vs. CY5
labeled Uncomplicated malaria so here ratio is between uncomplicated vs.
complicated i.e., CY3/CY5, but I also want to know what is the copy number
variation in  CY3 labeled sample and cy5 labeled sample individually
because both of them are disease conditions, so is there any way to analyze
this data,



Steps which I am following is:



·         I have taken g mean intensity of probes labeled with cy3 and cy5

·         Taken a standard deviation of  background mean intensities, then
multiplied this mean intensity with 2.6,

·         After multiplying added with bg mean intensity and then
subtracted with the gmean intensity

·         Is this the right manner of anlaysing this data

·         Or is there any other way out, since I am new to this I need to
know in which way should I proceed please help me out



              Array platform is Agilent array 44k and for only single
array, and please let me know is there any way to find out how many fold
the copy number variation is taking place





Regards

Isha Pandey

Email- isha.pandey@bits-pilani.ac.in, aahana10123@gmail.com

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