Dear Sir,

Thank you for your guidance. :)

I will proceed with my analysis.

Regards,

Rushiraj


On Thu, Oct 17, 2013 at 9:25 PM, Simon Anders <anders@embl.de> wrote:

> Hi
>
>
>  I have got a set of RNA-seq data with the control sample having around 8
>> million reads and the treated samples in the range of 13-17 million reads.
>>   I was wondering if DESeq can handle this kind of difference in number of
>> reads between samples?  Or a down sampling of reads is necessary before
>> DESeq?
>>
>
> No, there is no need to downsample. The statistical methods of DESeq and
> DESeq2 are designed to deal with this.
>
>   Simon
>
>
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