Good morning,

I work with mirnas analysis and I would like your help (if possible).
I have two set of tumor samples of human mirnas (N0 and N+, with and
without metastasis respectively) that were sequenced with SOLiD sequencer
and I would like to know if I there is a way to analyse differential
expression with Deseq. I saw that Deseq is usually used to compare paired
samples (for example, tumor and control of the same patient). I tried to
apply a example with my tests, using the nbinomTest function, but I didn't
get results (it returned NA)... Can anybody help me?

Thanks for your attention.

Best regards,

-- 
Liliane Santana
Bacharel em Ciência da Computação - UFS
Mestranda em Ciência da Computação - USP
Bioinformata - IIEP Albert Einstein

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