yes Gregory..


On Tue, Oct 1, 2013 at 2:36 PM, gregory voisin <voisingreg@yahoo.fr> wrote:

> Hi  if I understand well: you have 1 cel for the control and 1 cel for the
> experiment.
> You have no replication?
>
>
>
>
>   ------------------------------
>  *De :* deepika lakhwani <lakhwanideepika@gmail.com>
> *À :* bioconductor@r-project.org
> *Envoyé le :* Mardi 1 octobre 2013 19h27
> *Objet :* [BioC] Fwd: Regarding miRNA analysis
>
> hello...
>
> Can anyone clear one more thing about the miRNA analysis. As I mention
> earlier I have two miRNA cel files(rice) for one is control and other one
> is experiment . when I did analysis for differential miRNA using affylmgUI
> package. I got total 20707 mirna with significant and non significant P
> value.
> In this analysis, how can we identify that out of 20707 miRNA, which miRNA
> is hybridize properly.
>
>
>
> thanks
>
> regards
> deepika
>
>
> ---------- Forwarded message ----------
> From: deepika lakhwani <lakhwanideepika@gmail.com>
> Date: Tue, Oct 1, 2013 at 10:12 AM
> Subject: Regarding miRNA analysis
> To: bioconductor@r-project.org
>
>
> hello..
>
> I have miRNA hybridized CEL files for control and experiment. now i want to
> find out differential miRNA and also want to identify that how many miRNAs
> are hybridized. I do not know which package and method was used for miRNA
> analysis.
>
> I did Microarray analysis of RNA cel files and for that i used affylmGUI
> package and RMA method. but for the differential miRNA analysis same
> package can we used? If yes then i did the analysis and i got 20707 miRNAs
> with M and P values. Now i did not get that how many miRNAs are hybridized.
>
> I am so confused so Can any one help me in the field of micro array
> analysis?
>
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>
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