Hello Heidi,

I'm a real beginner in R and would like to analyze my miRNAs TLDA data
with your R-package.

I have 60 patients with 384*2 wells , I 'd like to use the following
features in order to make nice normalization with your software.

 

Well position     patient                 type of cancer  miRNA
Ct           

A1                          001                        CRC
hsa-miR-1           27.33     target

A2                          001                        CRC
has-miR-3           23.2       Endogenous Control

...

 

In the same folder I put a :

-          1 files.txt as mentioned in the description of the package
and put inside the description of the samples to analyze (i.e the
description of the patient and the corresponding .txt)

-          Each .txt of each samples which were described in the
files.txt.

 

I tried:

 

library("HTqPCR")

head(read.delim(file.path("D:/1-prestizia/laboratoire/protocole-stat-han
dbook/statistique/R/HTqPCR/test-gui/test-gui", "files.txt")))

files <-
read.delim(file.path("D:/1-prestizia/laboratoire/protocole-stat-handbook
/statistique/R/HTqPCR/test-gui/test-gui", "files.txt"))

 

and my problem is below! The readCtData function !!! impossible to open
the sample1.txt! """"No such file or directory""""

raw <- readCtData (files = files$File, path = path))

#show(raw)

 

Help J

Thank you in advance for your help!

Guillaume

 


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