Right but if you're injecting 10 mice with 5FU, injecting another 10 isn't
really that much marginal effort.  I'm sitting at a lab bench right now as
it happens :-)



On Mon, Aug 19, 2013 at 2:38 PM, Steve Lianoglou
<lianoglou.steve@gene.com>wrote:

> Hi,
>
> On Mon, Aug 19, 2013 at 2:30 PM, Tim Triche, Jr. <tim.triche@gmail.com>
> wrote:
> > http://arxiv.org/pdf/1301.5277v2.pdf may be of interest
> >
> > voom() transforms everything to log2(RPM) before estimating mean-variance
> > trends, so fractional count estimates from RSEM won't impact the modeling
> > anyhow.  See also
> >
> > http://www.statsci.org/smyth/pubs/VoomPreprint.pdf
> >
> > Personally I've wondered for a long, long time why people spend so much
> > time on GLMs for sensitivity, when more biological replicates would make
> > more difference.
>
> Money and time.
>
> Time to grow the mouse, I mean ... I wasn't referring to *your* time
> as a bioinformatician, no one actually cares about that as long as I
> can have the results by tomorrow, thanks! :-)
>
> -steve
>
> --
> Steve Lianoglou
> Computational Biologist
> Bioinformatics and Computational Biology
> Genentech
>



-- 
*He that would live in peace and at ease, *
*Must not speak all he knows, nor judge all he sees.*
*
*
Benjamin Franklin, Poor Richard's
Almanack<http://archive.org/details/poorrichardsalma00franrich>

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